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Chicken and ducks, the two major domestic poultry species, have been widely used as experimental animals in avian disease, comparative medicine and biological product safety and potent evaluation.In order to obtain better poultry vaccine, or clarify pathogenic mechanism of avian disease agents, laboratory birds are unavoidably suffered from experimental lethal challenge,which usually is considered as the cruelest and miserable activation on animals since the animals always end to death without any clinical treats.