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An antimicrobial substance R-1 produced by Brevibacillus laterosporus isolated from the sea sediment was purified by ultrafiltration,DEAE-Sepharose Fast flow chromatography,CM-Sepharose Fast flow chromatography and HPLC reversed phase column chromatography,and was characterized.The molecular weight (MW) was accurately determined by MALDI-TOF-MS as 1608.023 Da.And its pI was determined with Rotofor Cell BIO-RAD to be 8.55.