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Strawberry (Fragaria ananassa Duch.) production is increasing annually and China has the largest acreage planted in the world.Asexual reproduction material of strawberry once has been infected with the virus and then would spread widely through planting seedlings infected by virus or viruses.As viral disease occurred, a general quality decline of imported strawberry varieties appears after years of cultivation, which causing strawberry production reduced severely to 30% ~ 80% per year.Thus, according to the occurrence and development of viruses infecting strawberry, virus free asexual propagation materials is the key to prevent strawberry virus diseases.