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Garden asparagus (Asparagus officinalis) belongs to the genus Asparagus, which comprises more than 200 species.Some of the dioecious Asparagus.species were found to be cross-compatible and interspecific hybridization was carried out to introgress agricultural traits of wild Asparagus species into garden asparagus.To date, several molecular marker techniques have been employed to identify sex-linked markers of garden asparagus and we developed two male specific markers, Asp1T7sp (Nakayama et al.2006) and TRsts (Kanno et al.in preparation).