【摘 要】
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The objective is to analyze tissue specific expression of BnMAPKlgenes,and exploretheir characteristics and signaling pathway in wound and Pathogenetic fungi stress.Method On the basis of silico cloni
【机 构】
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西南大学农学与生物科技学院,重庆市油菜工程技术研究中心,重庆400715
【出 处】
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中国遗传学会第九次全国会员代表大会暨学术研讨会
论文部分内容阅读
The objective is to analyze tissue specific expression of BnMAPKlgenes,and exploretheir characteristics and signaling pathway in wound and Pathogenetic fungi stress.Method On the basis of silico cloning,the promoters of BnMAPKl were cloned.Tissuespecificities in various organs of BnMAPKI and mRNA expression characteristics after inducedwere analyzed by Quantitative real-time PCR (qPCR).Result The cloned promoter sequences ofBnMAPKI contain severalhormone and stress responsive elements. BnMAPKl expression couldbe induced by phytohormones methyl jasmonate(MeJA),salicylic acid(SA),abscisic acid(ABA),hydrogen peroxide(HZ氏)wound,and sclerotinia sclerotio、Further experiments showed thatPDF1.2gene was activated but PR-1 was suppressed after wound and S.sclerotinrum treatments,indicate that oilseed rape most likelythrough jasmonic acid(JA) signaling in response to woundand pathogenetic fungi stress.Conclusion BnMAPKI expression could be induced by severalphytohormones and its promoter contain several hormone and stress responsive elements.BnMAPKl may playan important role in resistance to a variety of stress in plants and oilseed rapemight be mediated by JA signaling pathway in response to wound and pathogenetic fimgi stress.
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