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An improved freeze casting method is proposed for the fabrication of aligned macroporous monolith as stationary phase for high speed chromatography.The fabrication process includes mainly two steps as follows.First,the monomer solution is pre-polymerized into colloids and then dispersed within the solvent phase.Secondly,the suspension is directionally frozen in liquid nitrogen and further solidified to direct the formation of aligned macroporous monolith.The following characterization indicates that the monolith possesses both the obvious aligned macropores and the diffusion pores.The aligned macroporous monolith is tried as stationary phase for the adsorption evaluation.It reveals that the equilibrium adsorption time is about 70 min and the maximum adsorption capacity reaches 33.85 mg mL-1 for IgG and 45.69 mg mL-1 for lysozyme,respectively.And,the dynamic adsorption capacity at 50%breakthrough point reaches about 25.5 mg mL-1 of lysozyme and 22.4 mg mL-1 of IgG,respectively.Additionally,both dynamic adsorption capacity and column efficiency exhibit no obvious reduction with increasing flow rates.All these results suggest that the aligned macropores have positive effect on improving the chromatographic performance.Besides,it is worth to note that the fabricated aligned macroporous monolith is applicable and can be extended from chromatography to catalysis.