In addition to vector control and malaria chemotherapy, immunological intervention will be needed to stop the resurgence of malaria.Transmission-blocking vaccine (TBV) is one of the new strategies for controlling malaria.It will be a major method to prevent from malaria if TBV is applicated together with other stages malaria vaccines and anti-malaria drugs.P25 is the major surface protein expressed on the zygote and ookinete of Plasmodium, which is well known as the important candidate for the TBV.In recent years,the specific protein P48 expressed on the surface of gametocytes as a transmission-blocking immune target has caused widespread interesting.Here, we have pursued a vaccination strategy, based on DNA immunization in mice with genes encoding two antigens present on the sexual stages of Plasmodium yoelii 17XL (P.y 17XL), Pys48and Pys25, to induce important antibodies.The DNA vaccines of Pys25 and Pys48 were able to induce high levels of antibodies in mice.The effective transmission blocking activity was confirmed by mosquito feeds and ookinete culture in vitro, but the transmission-blocking activity of Pys25 and Pys48 composite immune group was lower than that for either Pys25 or Pys48 simple immune group, moreover the effect has significant antibody titer dependent.Therefore, our research will provide necessary and basic data for identify the role of P25 and P48 in transmission-blocking immune and developing effective compound malaria vaccines.