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Traditional Chinese medicines have been used to treat human disease in China for centuries.TCMs and TCM formulations normally consist of multiple active components that come from different plants.The matrix of TCMs is often very complex; therefore, separation often requires a gradient method with a long period of time.The typical example is TCMs containing ginsenosides or notoginsenosides.The China Pharmacopeia method using conventional LC column takes almost 120 minutes to separate these compounds.