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目的:建立HPLC法测定濒危野生药用植物中华补血草中槲皮素和木犀草素含量的方法,为中华补血草的质量控制提供理论依据。方法:采用高效液相色谱法同时测定中华补血草中槲皮素和木犀草素的含量。色谱柱:TURNER Kromasil TM C18柱(250 mm×4.6 mm,5μm),流动相为甲醇:0.2%磷酸溶液(55∶45)进行等度洗脱,流速为1 ml·min-1,检测波长为360nm。结果:槲皮素在浓度0.92~92μg·ml-1范围内有良好的线形关系,相关系数r为0.999 6,平均加样回收率为98.87%,RSD为1.68%(n=6)。木犀草素在0.412~41.2μg·ml-1范围内有良好的线形关系,相关系数r为0.999 8,平均加样回收率为99.33%,RSD为1.89%(n=6)。结论:方法简便可行、灵敏准确,可作为我国沿海野生中华补血草质量评价的方法之一,该研究对于保存中华补血草野生品种相关资料,具有重要意义。
OBJECTIVE: To establish a HPLC method for the determination of quercetin and luteolin in the endangered wild medicinal plant Limonium, providing a theoretical basis for the quality control of Limonium sinense. Methods: Simultaneous determination of quercetin and luteolin in Limonium sinense by high performance liquid chromatography. Column: TURNER Kromasil TM C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase of methanol: 0.2% phosphoric acid (55:45) for isocratic elution at a flow rate of 1 ml · min- 360nm. RESULTS: Quercetin had a good linear relationship in the concentration range of 0.92-92 μg · ml-1 with a correlation coefficient r of 0.999 6. The average recovery was 98.87% with a RSD of 1.68% (n = 6). Luteolin in the range of 0.412 ~ 41.2μg · ml-1 has a good linear relationship, the correlation coefficient r was 0.999 8, the average recovery was 99.33%, RSD 1.89% (n = 6). Conclusion: The method is simple and feasible, sensitive and accurate, and can be used as one of the methods to evaluate the quality of wild Limonium sinense in China. The study is of great significance for preserving the related materials of Limonium sinense.