The white rot basidiomycete Pleurotus ostreatus is the third largest commercially cultivated edible mushroom in the world.During the growth, P.ostreatus is vulnerable to the threat of various diseases which will cause the loss of yield and quality seriously.Suppressor of G2 Allele of SKP 1 (SGT1), as an important regulatory element in variety of disease resistance signalling pathways, attracts a great deal of interest from breeding researchers.Previously, we acquired a 480bp fragment of SGT1 gene by suppression subtractive hybridization (SSH).