Recent studies suggest that the genus Trichoderma are economically important and of high species diversity.Correct identification becomes critical for theoretical and applied researches of this fungal group.For accurate and rapid species identification, three DNA marker, ITS, rpb2 and tef1, were selected and evaluated as candidate DNA barcodes for the genus.Adequate intra-and inter-specific variations and efficiency of PCR amplification and DAN sequencing are considered as important criteria.