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为研究p16和 p5 3基因的联合应用对胆管癌细胞的作用 ,将重组体腺病毒 p16、p5 3、p16联合 p5 3转移到人胆管癌细胞QBC939,对 p16、p5 3基因的表达、细胞的生长抑制及机制进行了分析。RT PCR显示在胆管癌QBC939细胞系中p16呈低表达 ,p5 3不表达。重组体腺病毒能介导 p16和p5 3等外源基因在胆管癌QBC939细胞系中高效表达 ,两者联合应用能明显抑制QBC939细胞的生长和集落形成。流式细胞计数证实其能诱导QBC939细胞发生明显凋亡并导致其发生G1期阻滞。本研究显示 p16及 p5 3基因通过诱导肿瘤细胞凋亡及G1期阻滞在肿瘤的基因治疗方面发挥作用 ,两者联合应用具有协同作用
In order to study the effect of combined use of p16 and p53 genes on cholangiocarcinoma cells, the recombinant adenoviruses p16, p53, p16 and p53 were transferred to human cholangiocarcinoma cells QBC939, and the expression of p16 and p53 genes, cells Growth inhibition and mechanism were analyzed. RT PCR showed low expression of p16 in the cholangiocarcinoma QBC939 cell line and no p53 expression. Recombinant adenovirus can mediate the exogenous gene expression of p16 and p53 in the cholangiocarcinoma QBC939 cell line. The combination of the two can significantly inhibit the growth and colony formation of QBC939 cells. Flow cytometry confirmed that it could induce significant apoptosis in QBC939 cells and lead to G1 arrest. This study showed that p16 and p53 genes play a role in the gene therapy of tumors by inducing apoptosis and G1 arrest in tumor cells, and the combination of them has a synergistic effect.