Objective: The expression of BLNK in tissues and serum of esophageal carcinoma patients was investigated.Methods: Bioinformatics analysis of BLNK was based on the NCBI database (http://www.ncbi.nlm.nih.gov/).RNA from seven primary esophageal carcinoma tissues and paired non-carcinoma tissues were extracted using TRIZOLTM agent.The expression of BLNK was detected by reverse transcriptionPCR and real-time PCR with GAPDH as an internal control.Genomic DNA from peripheral blood and circulating DNA from serum of esophageal carcinoma patients were extracted using the QIAamp(R) DNA Blood Kit and QIAamp(R) UltraSense Virus Kit,respectively.Microsatellite analysis of BLNK was carried out by fluorescence-labeled PCR and gene sequencing using GeneScan and Genotyper software.