FISH检测单精子AZFc微缺失研究

来源 :GTAUF2014 & APSMHA2014第六届长城泌尿男科转化医学论坛暨第七届亚太男性健康与抗衰老研究学会年会 | 被引量 : 0次 | 上传用户:kisswc69
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目的:FISH分析外周血AZF无缺失的严重少精子症患者精子AZFc缺失情况.方法:实验组包括16例外周血检测未见AZF缺失的严重少精子症患者,选择2例已经正常怀孕的男性作为阳性对照组,选取2例AZFc缺失的少精子症患者作为阴性对照.应用荧光原位杂交法检查精子中AZFc缺失情况,选用18号染色体着丝粒探针为参照,SRY作为Y染色体特异性信号,DAZ基因作为AZFc特异性信号.每组观察100个精子,分析血液中AZF无缺失的严重少精子症患者精子AZFc缺失情况.
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