目的:探讨改良额肌腱膜瓣悬吊术在治疗上睑下垂较传统额肌腱膜瓣悬吊术术中及术后优势.方法:选择我院2012年20例(26眼)年龄5-12岁行传统额肌腱膜瓣悬吊术和我院2013年24例(32眼)年龄5-12岁行改良额肌腱膜瓣悬吊术进行比较.结果:1、术后治愈率基本相同.2、手术时间明显缩短:改良额肌腱膜瓣悬吊术平均为35±10分钟,传统传统额肌腱膜瓣悬吊术均为60±10分钟.3、术中损伤周围组织及出血
目的 近年来,分子生物学技术的发展对肿瘤的早期诊断有很大推动作用,特别是在基因标志方面,有了很大的发展。本实验采用RT-PCR技术,通过Bhas42细胞转化试验检测22个与促长阶段相关基因在不同组别以及不同时间点表达的情况,这些基因全部与细胞分裂周期、调节转录、抗凋亡、正性调节细胞增殖有关,探索目标基因成为检测化合物致癌促长活性标志基因的可能性。方法 试验采用已知的促癌化合物佛波酯(TPA)作为阳
随着药物临床前安全评价机构发展和国际合作的日益增多,兽医功能在动物毒理实验过程和动物设施管理中职能分工越显明确,并逐步与国际同行接轨.在日常兽医能力建设和实践过程中不断总结和体会,认为下面几个方面对规范兽医工作,提高动物设施管理,保障动物质量有一定的借鉴意义.①保障动物质量符合实验要求.实验动物质量是动物实验最基本的保障,所以应将动物质量保障作为兽医工作的重中之重.
OBJECTIVE To assess the nephrotoxicity of imipenem and to correlate the pathogenesis of lesions in the kidney with changes in urinary biomarkers of kidney toxicity.METHODS Study #1, 11 monkeys (2-3/se
OBJECTIVE To evaluate whether three assays, respectively, for the measurement of human C-reactive protein (CRP), haptoglobin(Hp) and total bile acid (TBA) were suitable for the applications in cynomol
OBJECTIVE To investigatethe proliferation activity of alimentarycanal (AC)after irradiation exposure on rhesus monkeys, 5-bromo-2-deoxyuridine (BrdU) was employedfor identifyingproliferative cells ine
OBJECTIVE Moxifloxacin is a fluoroquinolone with a broad spectrum of antibacterial activity.The aim of this study was to investigate the cardiovascular effects of moxifloxacin, administered to conscio
Dog and monkey are frequently used for cardiovascular safety pharmacology and for toxicology studies, but are not often used for central nervous system (CNS) pharmacological efficacy and safety pharma
OBJECTIVE To demonstrate that the DSI/Ponemah Jet System (v5.00) can generate high quality ECG data with current methods, enabling cardiovascular function to be assessed in free moving dogs in their h
目的 结合多种免疫细胞功能试验,探讨人和食蟹猴外周血单核细胞免疫功能的差异,为临床前药物免疫毒性评价建立起合适的体外试验方法,并且为生物技术药物安全性评价选择动物种属及动物毒性研究外推之人的风险评估提供科学支持.方法 (1)单抗诱导细胞因子释放试验:采用了Lucy Findlay建立的风干包被法及湿包法,人和食蟹猴PBMC细胞与ANC28.1共孵育2h,24 h,48 h,然后进行TNF-α,IF