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Animal multi-functional cellulase could function as endo-1,4-β-D-glucanase,exo-1,4-β-D-glucanase and β-D-glucosidase.Designing primers,RT-PCR was employed to amplify a multi-functional cellulase gene (mfc) fiom the stomach tissue of Ampullaria crossean.DNA sequencing results showed that the amplified mfc had 1225bp including 19bp of 5 end of upstream un-translated region,21bp of 3 end of non-coding region,and 1185bp of an open reading frame.