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Previously, we found that CTCF play an important role in the coregulated imprin-ted genes, IGF2 and H19, and different methylation profiles for H19ICR were associated with aberrant imprinting of IGF2 and H19 in human hepatocellular carcinoma (HCC).We assume that CTCF would have an equally complex set of parameters in regulating genomic imprinting in human cancer.To this purpose, we sought to search for interaction proteins of CTCF.In a yeast two-hybrid screen, we identify a novel partner, VIGILIN, which has been characterized as a highly conserved multi-KH-domain protein that binds RNA and ssDNA.A specific interaction between CTCF and VIGILIN is confirmed in immunoprecipitation (IP) in human cells (HepG2 and CNE1).Overexpression of CTCF or VIGILIN downregulates IGF2 expression and enhances H19 expression in HepG2 and CNE1 cells.In contrast, reduction of CTCF or VIGILIN with specific shRNA results in an increase of IGF2 and a decrease of H19.