论文部分内容阅读
Objective:To explore the effect of Xiaohe granules and its influence on estrogen receptor(ER) and progesterone receptor(PR) level , epidermal growth factor receptor (EGFR) level, proliferation cell nuclear antigen（PCNA）level in galactophore tissues ,phathological changes in estrogen–induced hyperplasia of mammary glands model of rats.To discuss the drug’s mechanism for prevertion and treatment of the disease.
Methods: 60 unpregnant healthy female Wister rats were randomed into normal group ,model group,Xiaohe group and Tamoxifen(TAM)group,fifteen rats in each group,Except for normal group injected with saline ,all the other three groups were injected with estradiol and progesterone to induce hyperplasia of mammary glands models.The rats were killed and serum was separated from blood after eight weeks .The size of breast were measured,Pathological histological examination was performed on the mammary glands.The expression of ER、PR、PCNA、EGFR in mammary cell were measured by immunohistochemistry.
Results: The size of breast of rats by statistics,comparison between model group and Xiaohe group showed significant differences （P﹤0.01,P﹤0.05）.Pathological histological observation proved:rat’shyperplisia of mammary glands in the model group as diffuse,more acinus and small lobes,less fat and connective tissue,enlarged acinus cavity and gland duct.Compared with model group,the Xiaohe group showedmuch less acinus and lobes.Compared with normal group the number of ER 、PR、PCNA positive cell in model group was increased.Compared with model group ,Xiaohe group or TAM group show much less ER 、PR 、PCNA positive cell .
Conclusion: The experimental study suggests:Xiaohe granules has the efficacy on retraining the mammary glands caused by estradiol and progesterone. In aspect of treatment,Xiaohe group was much better than the TAM group. It can lower the big rat mammary gland tissue ER, the PR expression, make the mammary gland tissue descended to the sensitivity of the sex hormones, and can lower the expression of PCNA,the suppressor cell enzyme amplification, but have no influence to the EGFR.