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目的:研究微流控芯片精子优选技术对精子常规参数及DNA完整性的影响。方法:自行设计制作微流控芯片,利用芯片处理技术和上游法对40例精液标本进行精子优选,通过计算机辅助精液分析系统及染色质扩散试验从精子常规参数及DNA完整性两个方面评价精液体外处理对精子的影响。结果:精液经微流控芯片法和上游法处理后,精子活力、精子正常形态率以及精子尾部肿胀率均有显著提高(P<0.01),精子的DNA损伤率明显降低(P<0.01)。微流控芯片法与上游法相比,优选后前者精子DNA损伤率明显低于后者[(8.4±5.8)%vs(16.4±9.2)%,P<0.01],而其他参数差异无显著性。结论:微流控芯片技术在精子优选中能获得精子DNA损伤程度小的高质量精子。
Objective: To study the effect of microfluidic chip sperm optimization on sperm routine parameters and DNA integrity. Methods: Microfluidic chips were designed and produced by ourselves. 40 sperm samples were sperm optimized by chip processing technology and upstream method. The sperm motility was evaluated by computer aided semen analysis system and chromatin diffusion test from the aspects of sperm general parameters and DNA integrity Effects of in vitro treatment on sperm. Results: Sperm motility, normal sperm morphology and sperm tail swelling rate were significantly increased (P <0.01), and the DNA damage rate of sperm was significantly decreased (P <0.01) after treated by microfluidic chip method and upstream method. Compared with the upstream method, the DNA damage rate of the former sperm was significantly lower than that of the latter by microfluidic chip method [(8.4 ± 5.8)% vs (16.4 ± 9.2)%, P <0.01], while the other parameters had no significant difference. Conclusion: Microfluidic chip technology can obtain high quality sperm with less sperm DNA damage in sperm selection.