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目的探讨利多卡因（Ｌｉｄｏ）对内毒素（ＥＴ）激活的中性粒细胞（ＰＭＮ）致内皮细胞损伤的保护作用。方法利用体外培养的内皮细胞，建立ＥＴ－ＰＭＮ－血清（ＨＳ）致内皮细胞损伤的模型，选用原代培养的小牛主动脉内皮细胞，纯化后４～９代的细胞制成悬液，接种于培养板，随机分为三组：对照组（ＥＣ＋ＰＭＮ＋ＰＢＳ），损伤组（ＥＣ＋ＰＭＮ＋ＨＳ＋１０μｇ／ｍｌ ＥＴ）和 Ｌｉｄｏ组（ＥＣ＋ＰＭＮ＋ＨＳ＋１０μｇ／ｍｌＥＴ＋１０ｕｇ／ｍｌ Ｌｉｄｏ）；另设三组（１μｇ／ｍｌ、１０μｇ／ｍｌ和１００μｇ／ｍｌ Ｌｉｄｏ）比较不同浓度Ｌｉｄｏ对内皮细胞的增殖活力的影响；用比色法测定培养液中乳酸脱氢酶活性、丙二醛浓度，用ＭＴＴ法测定细胞增殖活力（ＭＴＴ ＯＤ值）。结果与对照组比较，两组细胞培养上清乳酸脱氢酶活性及丙二醛浓度明显增加（Ｐ＜０． ０５），ＰＭＮ与内皮细胞粘附率明显增加（Ｐ＜０． ０１），损伤组ＭＴＴ ＯＤ值明显降低（Ｐ＜０．０５）。与损伤组比较Ｌｉｄｏ组细胞培养上清乳酸脱氢酶活性及丙二醛浓度显著降低（Ｐ＜０．０５），低浓度（１μｇ／ｍｌ）和中浓度（１０μｇ／ｍｌ）Ｌｉｄｏ ＭＴＴ ＯＤ值明显增加（Ｐ＜０．０５）。结论一定浓度的利多?
Objective To investigate the protective effect of Lido on endothelial cell injury induced by endotoxin (ET) activated neutrophil (PMN). Methods ET-PMN-serum (HS) -induced endothelial cell injury was established by using endothelial cells cultured in vitro. Primary cultured endothelial cells of calf aorta were selected and cultured. (EC + PMN + HS + 10μg / ml ET) and Lido group (EC + PMN + HS + 10μg / mlET + 10μg / ml Lido). Three groups (1μg / ml, 10μg / ml and 100μg / Ml Lido) to compare the effect of different concentrations of Lido on the proliferation activity of endothelial cells. The activity of lactate dehydrogenase and the concentration of malondialdehyde in the culture medium were measured by colorimetric method. The MTT OD value was determined by MTT method. Results Compared with the control group, the lactate dehydrogenase activity and the concentration of malondialdehyde in the supernatant of the two groups were significantly increased (P <0.05), and the adhesion rate of PMN and endothelial cells was significantly increased (P <0.01) MTT OD value was significantly lower (P <0.05). Compared with the injury group, the activity of Lactate dehydrogenase (LDH) and malondialdehyde (MDA) in the cell culture supernatant of Lido group were significantly decreased (P <0.05), and the OD value of Lido MTT was significantly lower at low and medium concentrations (1μg / ml and 10μg / ml) Increase (P <0.05). Conclusion of a certain concentration of more profit?