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从胜利油田回注水中筛选得到一株硫酸盐还原菌,命名为zsz1209。经过16S rDNA序列分析,鉴定zsz1209为梭菌属(Clostridium)。实验研究了zsz1209的生理特性,并对通过调节环境pH来抑制菌株zsz1209生长繁殖的可行性进行了探讨。实验结果表明:菌株zsz1209的理想碳源为乙酸钠,在30～60℃之间可较好地生长;SO24-浓度低至50 mg/L时,生长未受到明显抑制。当培养环境pH高至8.5～9.0或低至3.5～4.0时,检测菌株zsz1209对SO24-和碳源(COD)的利用情况,发现zsz1209的生长受到明显抑制,结果表明利用改变环境pH来抑制微生物对油田的腐蚀具有可行性。
Sulfate-reducing bacteria were obtained from Shengli oil field injection water and named as zsz1209. After 16S rDNA sequence analysis, zsz1209 was identified as Clostridium. The physiological characteristics of zsz1209 were experimentally studied, and the feasibility of inhibiting the growth and propagation of strain zsz1209 by adjusting the pH of the environment was discussed. The experimental results show that the ideal carbon source of strain zsz1209 is sodium acetate, which can grow well from 30 to 60 ℃. The growth of the strain zsz1209 is not inhibited obviously when the concentration of SO24- is down to 50 mg / L. When the culture pH was as high as 8.5-9.0 or as low as 3.5-4.0, the strain zsz1209 was used to test the SO24- and carbon source (COD) utilization. The growth of zsz1209 was significantly inhibited. The results showed that by changing the environment pH to inhibit the growth of microorganisms Corrosion of the oil field is feasible.