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目的 MCP-1、CCR2在对乙酰氨基酚中毒急性肝损伤过程中的变化并探讨其可能的作用机制。方法高效液相色谱法(HPLC)测定血浆中对乙酰氨基酚浓度;ELISA方法动态检测血清中MCP-1、CCR2的浓度。结果 16例对乙酰氨基酚血药浓度达到中毒浓度,4小时后MCP-1、CCR2在血液中的表达明显增加,12小时后均有所下降,但变化不明显。结论高效液相色谱(HPLC)法能早期诊断乙酰氨基酚中毒;MCP-1、CCR2作为一对重要的炎症介质,可能参与了对乙酰氨基酚中毒急性肝损伤过程。
Objective To investigate the changes of MCP-1 and CCR2 in paracetamol-induced acute liver injury and to explore their possible mechanisms. Methods The concentration of paracetamol in plasma was determined by high performance liquid chromatography (HPLC). The concentrations of MCP-1 and CCR2 in serum were detected by ELISA. Results 16 cases of acetaminophen plasma concentration reached toxic concentration, 4 hours after MCP-1, CCR2 expression in the blood increased significantly after 12 hours have declined, but the change was not obvious. Conclusions High performance liquid chromatography (HPLC) can diagnose acetaminophen early. MCP-1 and CCR2, as important inflammatory mediators, may be involved in the process of acute hepatic injury induced by acetaminophen.