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测定了 21例烧伤患者外周血单个核细胞(MC)对 PHA的反应性。21例患者分成组Ⅰ(Ⅲ°面积≤10％)、组Ⅱ(Ⅲ°面积>10％)两组。组Ⅰ外周血 MC对PHA反应无显著改变;组Ⅱ在伤后 1～3天、伤后 2周时有显著降低(P<0.05,P<0.05)。去除 MC中的玻璃粘附细胞,两组对PHA反应都有增强趋势。提示烧伤后玻璃粘附的单核细胞可能有很强的免疫抑制作用。组Ⅱ患者的血浆抑制自身MC对PHA的反应性,但不抑制非粘附细胞(LC)的 PHA反应,提示烧伤后玻璃粘附的 MC可能介导自身血浆的免疫抑制作用。将体外分离出的玻璃粘附细胞加回到 LC中,反应无显著改变或反而增强。
The reactivity of peripheral blood mononuclear cells (MCs) to PHA in 21 patients with burn was measured. Twenty-one patients were divided into two groups: group Ⅰ (Ⅲ ° area ≤10%) and group Ⅱ (Ⅲ ° area> 10%). There was no significant change in the response of PHA to MCA in group I; group II was significantly reduced 1 to 3 days after injury and 2 weeks after injury (P <0.05, P <0.05). Removal of glassy adherent cells in MC both showed an increasing trend towards PHA. Prompted glass adhesion of monocytes after burn may have a strong immunosuppressive effect. The plasma of group Ⅱ inhibited the reactivity of MC to PHA, but did not inhibit the PHA of nonadherent cells (LC), suggesting that the adhesion of MC after burn may mediate the immunosuppression of plasma. Addition of vitreous adherent cells isolated in vitro to the LC did not change significantly or increased on the contrary.