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目的:探讨Aβ对大鼠学习记忆能力的影响及清心开窍方的防治作用。方法:将Aβ1-40注入大鼠双侧海马建立痴呆(AD)动物模型,动物实验采用对照观察法。治疗组与预防组用清心开窍方防治,对照组用双益平治疗,疗程结束后评定疗效,用Morris水迷宫法测试大鼠学习记忆能力;用化学比色法测定乙酰胆碱酯酶含量;采用放射免疫法测定乙酰胆碱转移酶和M受体的含量。结果:预防组和治疗组大鼠的学习记忆能力改善显著高于模型组(P<0.05)。脑内海马区ChaT和AchE活性,模型组ChaT活性显著降低,预防组和治疗组的ChaT活性升高,差异有显著意义(P<0.05)。对大脑皮层的M受体测定表明,模型组的M受体显著低于正常对照组,治疗组与预防组则有所上升。结论:清心开窍方能改善AD大鼠的学习记忆能力,对AD病具有预防和治疗作用。
Objective: To investigate the effect of Aβ on learning and memory of rats and the preventive and therapeutic effects of Qingxin Kaiqiao Decoction. METHODS: Animal model of dementia (AD) was established by injecting Aβ1-40 into the bilateral hippocampus of rats. The animal experiment was performed with a control observation method. The treatment and prevention groups were treated with Qingxin Kaiqiao Fang, the control group was treated with Shuangyi Ping, the curative effect was evaluated after the end of the treatment course, and the learning and memory abilities of rats were tested by Morris water maze test; the content of acetylcholinesterase was determined by chemical colorimetry; The content of acetylcholine transferase and M receptor was determined by immunoassay. Results: The improvement of learning and memory abilities in the preventive and treatment groups was significantly higher than that in the model group (P<0.05). The activities of ChaT and AchE in the hippocampus of the brain significantly decreased the activity of ChaT in the model group, and the activity of ChaT in the prophylaxis group and the treatment group increased (P<0.05). The measurement of M receptors in the cerebral cortex showed that the M receptors in the model group were significantly lower than those in the normal control group, while those in the treatment and prevention groups increased. Conclusion: Qingxin Kaiqiao Prescription can improve the learning and memory ability of AD rats and have a preventive and therapeutic effect on AD.