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目的:建立灵芝降糖胶囊的含量测定方法。方法:采用高效液相色谱法对制剂中的人参皂苷Rg1、Re、Rb1及三七皂苷R1进行定量测定。结果:人参皂苷Rg1在3.86～23.16μg范围内线性关系良好,r=0.999 9,平均回收率100.18%,RSD=1.52%;人参皂苷Re在0.628～3.768μg范围内线性关系良好,r=0.999 9,平均回收率100.22%,RSD=0.41%;人参皂苷Rb1在3.74～22.44μg范围内线性关系良好,r=0.999 9,平均回收率100.51%,RSD=0.74%;三七皂苷R1在0.764～4.584μg范围内线性关系良好,r=0.999 9,平均回收率99.05%,RSD=1.10%。结论:所建立的方法简便、快捷,重复性好,可用于该制剂的质量控制。
Objective: To establish a method for determination of Ganoderma Hypoglycemic Capsule. Methods: The ginsenosides Rg1, Re, Rb1 and notoginsenoside R1 in the preparation were quantitatively determined by high performance liquid chromatography. Results: The linear relationship of ginsenoside Rg1 in the range of 3.86 ~ 23.16μg was good, r = 0.999 9, the average recovery was 100.18% and the RSD was 1.52%. Ginsenoside Re had a good linear relationship in the range of 0.628 ~ 3.768μg with r = 0.999 9 , The average recovery was 100.22% and the RSD was 0.41%. The linear range of ginsenoside Rb1 was 3.74 ~ 22.44μg with r = 0.999 9, the average recovery was 100.51% and the RSD was 0.74% Good linearity in the range of μg, r = 0.999 9, with an average recovery of 99.05% and RSD = 1.10%. Conclusion: The established method is simple, rapid, reproducible and can be used for the quality control of the preparation.