[Objective] The aim was to optimize the sea cucumber peptide yield and its activity. [Method] The impact of VC, VE and leathin on the yield and activity of sea cucumber peptide were investigated by using Glutathione as the standard and 1,10-Phenanthroline method. [Result] Under the conditions of 0.1% VC , 0.2% VE and 2.0% leathin, the activity of sea cucumber peptide was about 50% high and the extraction yield of it could reach 7.12%. [Conclusion] The deep processing of sea cucumber provided effective study idea. [Objective] The aim was to optimize the sea cucumber peptide yield and its activity. [Method] The impact of VC, VE and leathin on the yield and activity of sea cucumber peptide were investigated by using Glutathione as the standard and 1,10- Phenanthroline method. [Result] Under the conditions of 0.1% VC, 0.2% VE and 2.0% leathin, the activity of sea cucumber peptide was about 50% high and the extraction yield could it could reach 7.12%. of sea cucumber provided effective study idea.