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目的分析miR-338-3p在肾透明细胞癌中的表达及其与凋亡的相关性,为深入探讨miR-338-3p在肾透明细胞癌中的作用机制提供理论基础。方法以曲古抑菌素A(Trichostatin A,TSA)诱导786-O及HK-2,应用荧光定量PCR法检测786-O及HK-2中miR-338-3p的表达水平,采用流式细胞术检测786-O及HK-2的细胞凋亡率。结果786-O细胞中miR-338-3p相对表达量显著低于HK-2细胞(P<0.01);786-O细胞中miR-338-3p的表达水平随着TSA浓度的升高而升高,HK-2细胞miR-338-3p的表达水平随着TSA浓度的升高而下调;786-O及HK-2的凋亡率均随着TSA浓度升高而升高。以不同浓度TSA诱导时,786-O细胞的凋亡率与miR-338-3p表达水平呈显著正相关,相关系数为0.985(P<0.01)。HK-2细胞凋亡率与miR-338-3p表达水平无显著相关性(P>0.05)。结论 miR-338-3p在肾透明细胞癌细胞中的表达水平显著低于正常细胞,肾透明细胞癌细胞的凋亡率与miR-338-3p表达水平显著正相关;miR-338-3p可能参与肾细胞癌的发生、发展,提示其具有作为肾细胞癌诊断和治疗靶点的潜能。
Objective To analyze the expression of miR-338-3p in renal clear cell carcinoma and its relationship with apoptosis, and to provide a theoretical basis for further study on the mechanism of miR-338-3p in renal clear cell carcinoma. Methods 786-O and HK-2 were induced by Trichostatin A (TSA). The expression of miR-338-3p in 786-O and HK-2 was detected by real-time PCR. The apoptosis rate of 786-O and HK-2 cells was measured. Results The relative expression of miR-338-3p in 786-O cells was significantly lower than that in HK-2 cells (P <0.01). The expression of miR-338-3p in 786-O cells increased with the increase of TSA concentration The expression of miR-338-3p in HK-2 cells was down-regulated with the increase of TSA concentration. The apoptosis rates of 786-O and HK-2 cells increased with the increase of TSA concentration. The apoptosis rate of 786-O cells was significantly and positively correlated with the expression of miR-338-3p with different concentration of TSA, the correlation coefficient was 0.985 (P <0.01). There was no significant correlation between the apoptosis rate of HK-2 cells and the expression of miR-338-3p (P> 0.05). Conclusions The expression of miR-338-3p in renal clear cell carcinoma cells is significantly lower than that in normal cells. The apoptosis rate of clear cell renal carcinoma cells is positively correlated with the expression of miR-338-3p. MiR-338-3p may be involved in The occurrence and development of renal cell carcinoma suggest its potential as a diagnostic and therapeutic target for renal cell carcinoma.