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目的:探索建立一种慢性点燃抗脑抗体癫癎动物模型。方法:在SD大鼠的海马CA1区间断持续注射抗脑抗体——神经突触前膜蛋白(Munc-18抗体)1μL,隔天注射一次,5次之后每隔2周注射一次,共10周。监测注射前后大鼠的脑电图(EEG)和癎样行为。3个月后取脑切片,HE和尼氏染色后镜下观察。结果:实验组12只大鼠中有10只有癎样EEG(83％),6周之后有5只(50％)保留;9只有1～5级癎样行为(75％),第6周后保留4只(44％);而所有对照组中,仅2只大鼠有轻度异常脑波和1只有2级癎样行为,相比有显著差异(P<0.01)。镜下发现实验组损伤灶明显,胶质细胞增生,神经元细胞减少且形态异常。结论:Munc-18抗体能慢性点燃致癎大鼠,但其作用机制需进一步研究。
OBJECTIVE: To establish a chronic animal model of anti-brain antibody epilepsy. Methods: 1 μL of anti-brain antibody - pre-synaptic membrane protein (Munc-18 antibody) was continuously injected into the hippocampal CA1 region of SD rats, injected once every other day and injected every 2 weeks after 5 times for 10 weeks . Electroencephalography (EEG) and 癎-like behavior of rats before and after injection were monitored. Three months later, brain sections were taken and observed under microscope after HE and Nissl staining. RESULTS: Ten of the 12 rats in the experimental group had eh-like EEG (83%), five (50%) remained after 6 weeks, nine had grade 1-5 (75%), and after 6 weeks 4 (44%) were retained. Only 2 rats in all control groups had mild abnormal brainwave and 1 grade 2 癎-like behavior, with significant difference (P <0.01). Microscopic examination showed that the lesion in the experimental group was obvious, the proliferation of glial cells, the decrease of neuronal cells and the abnormal morphology. Conclusion: Munc-18 antibody can cause chronic ignition in rats, but its mechanism needs further study.