论文部分内容阅读
目的:探讨人参皂苷Rh2联合顺铂对于人卵巢癌细胞株SKOV-3的影响。方法:采用MTT比色法检测人参皂苷Rh2与DDP联合应用对SKOV-3细胞株增殖的影响,利用流式细胞仪分析细胞周期及凋亡情况,并计算细胞平均凋亡率,利用Western-blot技术观察SKOV-3细胞内HER-2蛋白的表达。结果:①Rh2与DDP联合应用分别作用于SKOV-3卵巢癌细胞株,单独应用DDP 350μmol/L和600μmol/L IC50降为DDP 250μmol/L和300μmol/L。②流式细胞术检测显示,DDP+Rh2组凋亡率在SKOV-3细胞系中为32.2%,SKOV-3细胞被阻止于G1期,在SKOV-3 DDP细胞中为12.5%,均显著高于仅应用DDP组细胞(P<0.05),SKOV-3 DDP细胞亦被阻止于G1期。③Western blotting结果显示,SKOV-3细胞中DDP+Rh2组HER-2蛋白表达较对照组、DDP组、Rh2组显著降低(P<0.05)。结论:人参皂苷Rh2与DDP联合应用对SKOV-3卵巢癌细胞株有促凋亡作用,并改善卵巢癌细胞株SKOV-3 DDP对DDP的敏感性。
Objective: To investigate the effect of ginsenoside Rh2 combined with cisplatin on human ovarian cancer cell line SKOV-3. Methods: MTT colorimetric assay was used to detect the effects of ginsenoside Rh2 combined with DDP on the proliferation of SKOV-3 cells. The cell cycle and apoptosis were analyzed by flow cytometry. The average apoptosis rate was calculated by Western blot The technique was used to observe HER-2 protein expression in SKOV-3 cells. Results: ①The combination of Rh2 and DDP was applied to SKOV-3 ovarian cancer cell lines respectively, and the DDP concentrations of 250μmol / L and 300μmol / L were decreased with DDP 350μmol / L and 600μmol / L IC50 alone. ② Flow cytometry showed that the apoptotic rates of DDP + Rh2 group were 32.2% in SKOV-3 cell line, SKOV-3 cells were blocked in G1 phase and 12.5% in SKOV-3 DDP cells In DDP group alone (P <0.05), SKOV-3 DDP cells were also blocked in G1 phase. ③Western blotting results showed that HER-2 protein expression in DDP + Rh2 group was significantly lower than that in control group, DDP group and Rh2 group (P <0.05). Conclusion: The combination of ginsenoside Rh2 and DDP can promote the apoptosis of SKOV-3 ovarian cancer cell line and improve the sensitivity of ovarian cancer cell line SKOV-3 DDP to DDP.