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目的 :为了解抗粘放菌 Ⅰ型菌毛单抗的抗粘附机理 ,在提取和鉴定抗粘放菌 Ⅰ型菌毛单克隆抗体和多克隆抗体的基础上 ,进行了抗粘附作用的研究。方法 :实验采用体外抗体阻断粘放菌 5 5 19和 5 95 1对牙釉质片的粘附 ,并结合扫描电镜和Ca+ + 测定方法进行。结果 :结果显示 ,单克隆和多克隆抗体能有效地阻断粘放菌 5 5 19对牙釉质片的粘附。扫描电镜发现未经抗体处理的牙釉质片表面粘附大量的细菌 ,而经抗体处理的牙釉质片表面细菌粘附明显减少 ,与牙釉质表面脱矿程度及钙离子浓度差异结果相吻合。结论 :这些结果提示 ,Ⅰ 型菌毛主要介导细菌对牙釉质片的粘附 ,抗 Ⅰ型菌毛的抗体可以有效地阻断细菌的粘附
OBJECTIVE: To understand the anti-adhesion mechanism of antimycobacterial type I pili mock anti-adhesion and to identify and characterize the anti-adhesion bacteria type I pili monoclonal and polyclonal antibodies the study. METHODS: In vitro experiments were performed to block the adhesion of adhesive smears 5 5 19 and 5 95 1 to enamel pieces. The results were combined with scanning electron microscopy and Ca + determination. Results: The results showed that monoclonal and polyclonal antibodies could effectively block the adhesion of 519 to the enamel patch. Scanning electron microscopy showed that a large number of bacteria adhered to the surface of enamel without antibody treatment. However, the bacterial adhesion on the surface of enamel treated with antibody was significantly reduced, which was in good agreement with the degree of demineralization and calcium concentration in enamel. CONCLUSIONS: These results suggest that Type I pilus mainly mediates the adhesion of bacteria to enamel pieces and that antibodies against Type I pilus effectively block bacterial adhesion