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目的探讨双氢青蒿素(DHA)对癌细胞和正常细胞的毒性差异,为将青蒿素类药物用于肿瘤的治疗提供实验依据。方法以来源于肺组织的癌细胞A549和正常支气管上皮HBE细胞为研究对象,采用噻唑蓝比色(MTT)、集落形成、吖啶橙/溴化乙锭(AO/EB)双染、蛋白浓度测定、乳酸脱氢酶(LDH)释放5种不同的细胞毒性实验观察DHA对2种细胞的毒性差异。结果不同浓度的DHA处理24 h后,2种细胞存活率均显著降低,DHA对A549和HBE细胞的半数抑制浓度分别为61.55μmol/L和87.83μmol/L。与自身对照组(0μmol/L DHA)相比,DHA(20和60μmol/L)可以减少A549和HBE细胞的集落形成率、蛋白含量,同时增加细胞死亡率和LDH释放水平(P<0.05),这些效应与DHA浓度具有一定相关性,且A549细胞的效应高于HBE细胞(P<0.05)。结论 DHA对来源于肺组织的癌细胞和正常细胞均有明显的细胞毒性,且以A549细胞敏感,这对DHA用于肿瘤的治疗具有积极的意义,但同时不能忽视DHA对正常细胞的毒副作用。
Objective To investigate the toxicity of dihydroartemisinin (DHA) to cancer cells and normal cells, and to provide experimental evidence for using artemisinin in the treatment of tumors. Methods Lung cancer cells A549 and normal bronchial epithelial cells were used as research objects. MTT, AO / EB staining, acridine orange / ethidium bromide (EB) staining, protein concentration 5 different cytotoxity experiments were performed to determine the toxicity of DHA to the two kinds of cells by measuring the release of lactate dehydrogenase (LDH). Results After treated with different concentrations of DHA for 24 h, the viability of both cell lines was significantly decreased. The half inhibitory concentrations of DHA on A549 and HBE cells were 61.55 μmol / L and 87.83 μmol / L, respectively. DHA (20 and 60 μmol / L) decreased the colony formation rate and protein content of A549 and HBE cells compared with their control (0 μmol / L DHA), while increased the cell death rate and LDH release (P <0.05) These effects were correlated with DHA concentration, and the effect of A549 cells was higher than that of HBE cells (P <0.05). Conclusion DHA is obviously cytotoxic to cancer cells and normal cells derived from lung tissue and sensitive to A549 cells, which is of positive significance for the treatment of cancer with DHA. At the same time, DHA can not ignore the toxic and side effects of DHA on normal cells .