论文部分内容阅读
目的研究石家庄市灌溉用污水对大鼠肝功能的影响,以期为污水灌溉的安全性评价提供参考。方法采用气相色谱-质谱联用仪和电感耦合等离子体质谱仪测定石家庄市农田污灌水样中的重金属含量和有机污染物成分。将80只SD大鼠随机分成4组,每组20只,雌雄各半,其中3个染毒组分别为25%,50%及100%污水,采用自由饮水方式进行染毒,连续28 d;并以蒸馏水为阴性对照组。分别于染毒前及染毒第7、28天采集大鼠血样,通过全自动生化分析仪测定血清中天门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活力。结果水样中铬和铅含量分别超过国标0.78、0.43倍,共检出30种有机化学物,主要为多环芳烃类化合物、邻苯二甲酸酯类、杂环类化合物及苯胺类化合物等。与对照组相比,饮用灌溉用污水28 d时,50%和100%剂量组雄、雌性大鼠血清ALT活力升高,差异有统计学意义(P<0.05)。结论石家庄灌溉用污水可引起大鼠肝损伤。
Objective To study the effect of irrigation wastewater on rat liver function in Shijiazhuang City in order to provide reference for the safety evaluation of sewage irrigation. Methods Gas chromatography-mass spectrometry (GC-MS) and inductively coupled plasma mass spectrometry (ICP-MS) were used to determine the contents of heavy metals and organic pollutants in sewage water samples from farmland in Shijiazhuang. Eighty SD rats were randomly divided into 4 groups with 20 mice in each group, half male and half female, and 3 of them were exposed to 25%, 50% and 100% respectively. The rats were exposed to free water for 28 days. And distilled water as negative control group. The blood samples of rats were collected before exposure and on the 7th and 28th day of exposure respectively. The activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured by automatic biochemical analyzer. Results The contents of chromium and lead in the water sample were 0.78 and 0.43 times higher than the national standard, respectively. A total of 30 kinds of organic chemicals were detected, mainly polycyclic aromatic hydrocarbons, phthalates, heterocyclic compounds and aniline compounds. Compared with the control group, serum ALT activity of male and female rats in 50% and 100% dose groups increased significantly after drinking water for 28 days, with statistical significance (P <0.05). Conclusion Shijiazhuang irrigation water can cause liver injury in rats.