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目的:研究鸡矢藤环烯醚萜总苷(Iridoid glycosides of paederia scandens,IGPS)的镇痛作用及其机制。方法:采用小鼠福尔马林实验、扭体实验研究IGPS镇痛活性,通过小鼠竖尾实验和小鼠跳跃实验研究其成瘾性,通过纳洛酮拮抗实验和预先给予一氧化氮供体左旋精氨酸(L-Arginine,L-Arg)和一氧化氮合酶抑制剂N-硝基-L-精氨酸甲酯(N-nitro-L-arginine methy1ester,L-NAME)初步探讨IGPS镇痛作用与阿片受体和一氧化氮(Nitric oxide,NO)系统的关系。结果:IGPS(360、180mg/kg ig qd×7d)可显著抑制福尔马林实验Ⅰ相和Ⅱ相反应;IGPS(360、180、90mg/kg ig qd×7d)可明显抑制冰醋酸导致的小鼠扭体反应;IGPS(360mg/kg sc q12h×8d)连续给药动物均未出现S行竖尾反应及跳跃现象;纳洛酮(5mg/kg)不能拮抗IGPS镇痛活性,L-Arg(400mg/kg)可部分地抑制其镇痛作用,L-NAME(37.5mg/kg)可增强其镇痛作用。结论:IGPS具有明显的镇痛作用,且连续用药无成瘾性,其镇痛作用可能与内源性阿片肽系统无关,而与抑制NO的生成有关。
Objective: To study the analgesic effect of Iridoid glycosides of paederia scandens (IGPS) and its mechanism. METHODS: The IGPS analgesic activity was studied using mouse formalin test and writhing test. The addiction was studied by the mouse tail-tail experiment and mouse jump experiment, and the naloxone antagonist experiment and nitric oxide pre-administration were provided. A preliminary study on L-arginine (L-Arg) and nitric oxide synthase inhibitor N-nitro-L-arginine methyester (L-NAME) The relationship between IGPS analgesia and opioid receptor and nitric oxide (NO) system. Results: IGPS (360, 180 mg/kg ig qd×7d) significantly inhibited phase I and II responses in formalin; IGPS (360, 180, 90 mg/kg ig qd×7d) significantly inhibited glacial acetic acid. Twisting response in mice; No animals with IGPS (360mg/kg sc q12h x 8d) continuously received S-tail reaction and skipping; Naloxone (5mg/kg) did not antagonize IGPS analgesic activity, L-Arg (400mg/kg) can partially inhibit its analgesic effect, L-NAME (37.5mg/kg) can enhance its analgesic effect. Conclusion: IGPS has obvious analgesic effect, and continuous medication has no addiction. The analgesic effect of IGPS may not be related to endogenous opioid peptide system, but related to inhibition of NO production.