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国内首次发现的两例人体棘阿米巴角膜炎样品在作者所在实验室进行了形态学特征分析和致病性实验。这两个分离株(L和W)的形态特征极其相似:滋养体平均长度为27.5μm,平均宽度为21.0μm。包囊的平均直径为13.60μm;外囊贴近内囊,内囊呈多边形,但趋近圆形,内囊臂数为5—7;蛋白银染色显示不存在网状结构。两虫株对小鼠均有致病力。根据以上特征,作者将这两个中国人体致病性虫株初步鉴定为棘阿米巴属类群2中的Acanthamoebalugdunensis-A.quinacomplex.
The first human case of Acanthamoeba keratitis in our country was analyzed in morphological characteristics and pathogenicity in our laboratory. Morphological characteristics of these two isolates (L and W) are very similar: trophozoites average length 27.5 μm, average width 21.0 μm. The average diameter of the capsule was 13.60μm. The outer capsule was close to the inner capsule, the inner capsule was polygonal, but approached the circle with the number of inner capsule 5-7. The silver staining showed no network structure. Two strains of mice have pathogenicity in mice. According to the above characteristics, the two Chinese human pathogenic strains initially identified as Acanthamoeba group 2 Acanthamoebalugdunensis-A. quinacomplex.