论文部分内容阅读
目的探讨代谢综合征(MS)患者过氧化物酶体增殖物激活受体δ(PPARδ)+294T/C基因多态性与血脂、肥胖和左室肥厚的关系。方法检测300例MS、174例高血压病(EH)和143例2型糖尿病(DM)患者的体重指数(BMI)、腰围、血压、血脂、空腹血糖(FBG)和空腹血浆胰岛素(FINS)。MS诊断根据1999年WHO亚太诊断标准,其中389例患者行超声心动图检测心脏结构改变,应用聚合酶链反应-限制性片段长度多态性方法测定PPARδ+294T/C基因多态性。结果PPARδ+294T/C基因多态性各基因型频率分布组间比较差异无统计学意义。MS组血浆总胆固醇、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)水平和BMI明显高于DM组。MS组和EH组的左室重量(LVM)、左室重量指数(LVMI)和左室肥厚罹患率均明显高于DM组。MS组CC型血浆总胆固醇和LDL-C水平明显高于TT型和TC型[总胆固醇:CC型(6.13±1.86)mmol/L比TC型(5.14±1.10)mmol/L或TT型(4.99±1.42mmol/L),P<0.05或P<0.01;LDL-C:CC型(3.82±1.52)mmol/L比TC型(3.14±0.88)mmol/L或TT型(2.90±0.87)mmol/L,P<0.05或P<0.01]。分析PPARδ各基因型与LVMI和BMI的关系,发现MS组C等位基因携带者(CC+TC)LVMI和BMI明显高于TT型[LVMI:CC+TC(46±10)g/m2.7比TT(44±10)g/m2.7;BMI:CC+TC(26±3)kg/m2比TT(25±3)kg/m2,P<0·05]。结论MS患者PPARδ+294T/C基因多态性与肥胖和脂质紊乱关系密切,C等位基因携带者较TT基因型患者左室重构明显。
Objective To investigate the relationship between peroxisome proliferator - activated receptor δ (PPARδ) + 294T / C polymorphism and serum lipids, obesity and left ventricular hypertrophy in patients with metabolic syndrome. Methods Body mass index (BMI), waist circumference, blood pressure, blood lipid, fasting blood glucose (FBG) and fasting plasma insulin (FINS) were measured in 300 MS, 174 with essential hypertension (EH) and 143 with type 2 diabetes mellitus. MS diagnosis According to WHO 1999 Asia-Pacific diagnostic criteria, 389 patients underwent echocardiography to detect cardiac structural changes, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to determine the PPARδ + 294T / C polymorphism. Results There was no significant difference in frequency distribution between PPARδ + 294T / C polymorphism genotypes. The levels of total cholesterol, triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and BMI in MS group were significantly higher than those in DM group. The left ventricular mass (LVM), left ventricular mass index (LVMI) and left ventricular hypertrophy in MS and EH groups were significantly higher than those in DM group. The levels of total cholesterol (CCL) and LDL-C in CC group were significantly higher than those in TT group and TC group [total cholesterol: CC (6.13 ± 1.86) mmol / L vs 5.14 ± 1.10 mmol / L or TT (3.14 ± 0.88) mmol / L or 2.90 ± 0.87 mmol / L compared with TC group (3.82 ± 1.52 mmol / L) L, P <0.05 or P <0.01]. Analysis of the relationship between PPARδ genotypes and LVMI and BMI showed that LVMI and BMI of C + C carriers in CC group were significantly higher than those in TT group [LVMI: CC + TC (46 ± 10) g / m2.7 (44 ± 10) g / m2.7; BMI: CC + TC (26 ± 3) kg / m2 vs TT (25 ± 3) kg / m2, P <0.05). Conclusion The polymorphism of PPARδ + 294T / C gene in MS patients is closely related to the obesity and lipid disorders. The C allele carriers have significantly more left ventricular remodeling than TT genotypes.