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目的:检测PTEN、STAT3和CyclinD1蛋白在宫颈癌组织中的表达。方法:收集2009年1月～2011年1月经手术治疗的宫颈癌手术切除标本85例,所选病例按照TNM分期分为Ⅰ、Ⅱ、Ⅲ期,另取因子宫肌瘤而行子宫切除的正常宫颈上皮组织作为对照组;免疫组化法检测宫颈组织中PTEN、STAT3和CyclinD1蛋白的表达。结果:PTEN蛋白在宫颈癌组织中的阳性率为42.4%,明显低于正常对照组的95.0%(P<0.01);而STAT3和CyclinD1蛋白在癌组织中的阳性率分别为71.8%和83.5%,明显高于正常宫颈组织的15.0%和25.0%(P<0.01)。在3个临床分期中,PTEN、STAT3和CyclinD1蛋白的表达率均存在统计学差异(P<0.01),PTEN蛋白的表达量会随着临床分期的增加而减少,但STAT3和CyclinD1蛋白随着临床分期的增加其表达率也相应升高。结论:PTEN蛋白的表达降低和STAT3、CyclinD1蛋白的表达增加在宫颈癌的发生发展中起到一定的作用,可以用于肿瘤良、恶性的鉴别诊断、病理分级以及预后判断等方面。
Objective: To detect the expression of PTEN, STAT3 and CyclinD1 in cervical cancer. Methods: Totally 85 cases of cervical cancer surgically treated from January 2009 to January 2011 were collected. The selected cases were divided into Ⅰ, Ⅱ and Ⅲ stages according to TNM stage. The other cases were normal hysterectomy due to uterine fibroids Cervical epithelial tissue served as a control group; immunohistochemistry was used to detect the expression of PTEN, STAT3 and CyclinD1 in cervical tissues. Results: The positive rate of PTEN protein in cervical cancer was 42.4%, which was significantly lower than that in normal control (95.0%, P <0.01). The positive rates of STAT3 and CyclinD1 in cancer tissues were 71.8% and 83.5% , Which was significantly higher than that of normal cervical tissue (15.0% and 25.0%, P <0.01). The expression rates of PTEN, STAT3 and CyclinD1 in three clinical stages were significantly different (P <0.01). The expression of PTEN protein decreased with the increase of clinical stage. However, the expression of STAT3 and CyclinD1 protein in clinical stage Staging increased its expression rate also increased accordingly. Conclusion: The decreased expression of PTEN protein and the increased expression of STAT3 and CyclinD1 protein play an important role in the occurrence and development of cervical cancer. It can be used in the differential diagnosis of benign and malignant tumors, pathological grading and prognosis.