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目的:建立HPLC同时测定复方黄葵颗粒中毛蕊异黄酮葡萄糖苷、金丝桃苷、芦丁、异槲皮苷、杨梅素、槲皮素、马钱苷、莫诺苷的方法。方法:采用Aglient C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.05%甲酸水溶液为流动相进行梯度洗脱,流速0.8 m L·min-1,检测波长分别为240,260,360 nm,柱温30℃。结果:毛蕊异黄酮葡萄糖苷、金丝桃苷、芦丁、异槲皮苷、杨梅素、槲皮素、莫诺苷、马钱苷线性范围分别为3.5~35.0(r=0.999 6),32.09~320.9(r=0.999 7),2.21~22.12(r=0.999 7),18.68~186.9(r=0.999 8),6.32~63.20(r=0.999 7),3.54~35.44(r=0.999 6),12.39~123.9(r=0.999 6),12.88~128.8 mg·L-1(r=0.999 6)。平均回收率分别为98.1%(RSD 1.7%),99.7%(RSD 0.6%),98.7%(RSD 2.5%),100.1%(RSD 1.4%),99.7%(RSD 1.0%),99.9%(RSD 2.7%),99.0%(RSD 1.2%),99.7%(RSD 1.3%)。结论:所建立方法简便、准确,重复性好,可作为复方黄葵颗粒的质量控制。
Objective: To establish a HPLC method for simultaneous determination of calycosin, hyperin, rutin, isoquercitrin, myricetin, quercetin, loganin and morroniside in compound Huang Kui granules. Methods: Aglient C18 column (4.6 mm × 250 mm, 5 μm) was used to elute gradient acetonitrile-0.05% formic acid as mobile phase at a flow rate of 0.8 m L · min-1. The detection wavelength was 240, 260 and 360 nm, 30 ° C. Results: The linear ranges of calycosin, hyperin, rutin, isoquercetin, myricetin, quercetin, morroniside and loganin were 3.5 ~ 35.0 (r = 0.999 6), 32.09 ~ 320.9 (r = 0.999 7), 2.21-22.12 (r = 0.999 7), 18.68-186.9 (r = 0.999 8), 6.32-63.20 (r = 0.999 7), 3.54-35.44 (r = 0.999 6) 123.9 (r = 0.999 6), 12.88 ~ 128.8 mg · L-1 (r = 0.999 6). The average recoveries were 98.1% (RSD 1.7%), 99.7% (RSD 0.6%), 98.7% (RSD 2.5%), 100.1% (RSD 1.4%), 99.7% (RSD 1.0%), 99.9% %), 99.0% (RSD 1.2%), 99.7% (RSD 1.3%). Conclusion: The established method is simple, accurate and reproducible, which can be used as the quality control of compound Huang Kui granules.