The method of residue analysis of a new synthesized fungicide 2-allylphenol was studied by simulating the active compound structure in Gingko tree( Gingko biloba L. ) and its dissipation rate and terminal residue levels in tomato under field condition. Residues of 2-allylphenol were extracted from tomato matrix with acetone, purified by liquid-liquid extraction and Florisil cartrieges, and then determined by HPLC with UV-detector. The minimum detectable amount of 2-allylphenol was 3 × 10-9 g, the minimum detectable concentration of 2-allylphenol in the samples of tomato were 0.01 mg/kg. The ranges of average recoveries and coefficient variation of the method were 87.7%-90.2% and 1.25%-2.06%, respectively. The dissipation rate and terminal residue levels in tomato were determined with the method described above. The results showed that the half-life of 2-allylphenol in tomato was 6.37 d, and 2-allylphenol declined with 82.6 %of the initial deposit remaining in tomato at harvest. The terminal residue levels in tomato were 0.15 mg/kg and 0.20 mg/kg following the recommended doses and time intervals.