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目的探讨急性缺血性脑血管病血小板肌球蛋白轻链激酶（ＭＬＣＫ）和Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活性的变化及钙拮抗剂对Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活性的影响。方法用３２Ｐ同位素掺入法和比色法分别测定５８例脑中风病人，包括２２例短暂性脑缺血发作（ＴＩＡ）患者和３６例脑梗塞患者以及３５名健康对照者血小板ＭＬＣＫ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活性。结果ＴＩＡ组和脑梗塞组ＭＬＣＫ活性与对照组相比均有明显增加（Ｐ＜０．０１），而Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活性均低于对照组（Ｐ＜０．０５，Ｐ＜０．０１）。在ＴＩＡ组和脑梗塞组之间，这两种酶活性无明显差异（Ｐ＞０．０５）。用钙拮抗剂ｎｉｍｏｄｉｐｉｎｅ治疗后，ＴＩＡ组和脑梗塞组患者Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活性均有部分恢复（Ｐ＜０．０５）。结论血小板ＭＬＣＫ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活性与急性缺血性脑血管病的发生有密切关系，钙拮抗剂可部分改善血小板Ｃａ＋２，Ｍｇ２＋－ＡＴＰ酶活性状态。
Objective To investigate the changes of platelet myosin light chain kinase (MLCK) and Ca2 +, Mg2 + -ATPase activity in acute ischemic cerebrovascular disease and the effects of calcium antagonists on Ca2 +, Mg2 + -ATPase activity. Methods Fifty-eight stroke patients, including 22 transient ischemic attacks (TIA), 36 cerebral infarction patients and 35 healthy controls, were assayed for platelet MLCK and Ca2 +, Mg2 + -ATPase activity. Results The activity of MLCK in TIA group and cerebral infarction group were significantly higher than those in control group (P <0.01), while the activity of Ca2 + and Mg2 + -ATP was lower than that in control group (P <0.05, P <0.01). 01). There was no significant difference in activity between TIA group and cerebral infarction group (P> 0.05). After treatment with calcium antagonist nimodipine, the activity of Ca2 +, Mg2 + -ATP in TIA group and cerebral infarction group were partially recovered (P <0.05). Conclusion Platelet MLCK and Ca2 +, Mg2 + -ATPase activity are closely related to the occurrence of acute ischemic cerebrovascular disease. Calcium antagonists can partially improve the activity of platelet Ca 2+, Mg 2+ -ATPase.