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用ＢａｍＨＩ和ＨｉｎｄＩＩ将丙肝病毒Ｃ＋Ｅ１ＤＮＡ片段从其克隆载体ｐＧＥＭ３ｚｆ－ＨＣＶ／Ｃ＋Ｅ１上切下，经Ｔａｑ酶补齐３’末端后插入到载体ｐＳＶＬ－Ｔ中，构建成丙肝病毒Ｃ＋Ｅ１真核表达载体ｐＳＶＬ－ＨＣＶ／Ｃ＋Ｅ１。本实验中重组效率达６４．７％（１１／１７），正向插入为５０％（２／４）。
The C + E1 DNA fragment of Hepatitis C virus was excised from its cloning vector pGEM3zf-HCV / C + E1 with BamHI and HindII and inserted into the vector pSVL-T after Taq polymerase complementation of the 3 ’end to construct the C + E1 eukaryotic expression vector pSVL- HCV / C + E1. The efficiency of recombination in this experiment was 64.7% (11/17) and positive insertion was 50% (2/4).