The present study evaluated the potential of white-rot fungal strain Coriolus versicolor to decolorize five structurally different dyes in sequential batch reactors under optimized conditions. The experiments were run continuously for seven cycles of 8 d each. High decolorizing activity was observed even during the repeated reuse of the fungus, especially when the old medium was replaced with fresh medium after every cycle. Biodegradation was the dominating factor as the fungus was able to produce the enzyme laccase mainly, to mineralize synthetic dyes. The nutrients and composition of the medium played an important role in sustaining the decolorisation potential of the fungus. Ccob was found be an easy and cheap substitute for carbon source for the fungus. Glucose consumption by the fungus was in accordance to its decolorisation activity and chemical oxygen demand (COD) reduction.