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目的 获得并鉴定日本血吸虫 6 2 k Da重组抗原。方法 将 Sj6 2 / p GEX- 1λT/ TG2 克隆菌用 IPTG诱导表达 ,经超声粉碎 ,离心后取上清过 GS4B柱 ,用还原型谷胱甘肽洗脱液洗脱后获得纯化的 r Sj6 2 / 2 6 GST重组融合蛋白 ,用 Throm bin酶切后获得纯化的 r Sj6 2重组蛋白 ,并对重组蛋白进行 SDS- PAGE和 Western blotting鉴定。结果 重组蛋白纯度较高 ,且可被血吸虫感染的鼠血清识别。结论 用重组的方法获得了与天然虫源蛋白性质相似的重组抗原蛋白
Objective To obtain and identify the 6 2 kDa recombinant antigen of Schistosoma japonicum. Methods The Sj6 2 / p GEX-1λT / TG2 colonies were induced by IPTG and disrupted by sonication. After centrifugation, the supernatant was passed through a GS4B column and the purified rSj6 2 was eluted with reduced glutathione eluate / 2 6 GST recombinant fusion protein, digested with Throm bin to obtain purified r Sj6 2 recombinant protein, and the recombinant protein was identified by SDS-PAGE and Western blotting. Results The recombinant protein was highly purified and was recognized by the serum of schistosome infected mice. Conclusion Recombinant antigen proteins with similar properties to natural insect-derived proteins were obtained by recombinant method