论文部分内容阅读
目的观察大鼠脑挫伤后脑及重要器官组织HSP70的表达变化。方法健康SD大鼠45只,随机分为正常对照组(5只)、假手术组(5只)、损伤组(35只),损伤组按照伤后处死时间不同再分为1h、3h、6h、12h、1d、3d、7d等7个组,每组5只。采用自由落体法制作脑挫裂伤模型,对脑及重要器官组织取材进行HSP70免疫组织化学染色,结果进行图像分析。结果大鼠脑挫伤后1h,脑及心、肝、肺、肾、脾组织即可见HSP70免疫组化阳性细胞,且随伤后时间的延长,阳性染色细胞逐渐增多,于12h达高峰,然后开始下降,7d接近正常;图像分析结果显示脑及心、肝、肺、肾、脾组织阳性信号积分光密度(IOD)值的变化与前述变化规律相似,经统计学处理损伤组与对照组之间存在显著差异(P<0.05)。结论大鼠脑挫伤后,脑及重要器官组织HSP70的表达及其随时间变化趋势相同。
Objective To observe the expression of HSP70 in brain and vital organs after contusion in rats. Methods Forty-five healthy SD rats were randomly divided into normal control group (n = 5), sham operation group (n = 5) and injury group (n = 35). The injured group was further divided into 1h, 3h and 6h , 12h, 1d, 3d, 7d and other 7 groups, 5 in each group. The model of cerebral contusion and laceration was made by free-fall method. HSP70 immunohistochemical staining was performed on the brain and vital organs, and the results were analyzed. Results HSP70 immunohistochemical positive cells were found in brain, heart, liver, lung, kidney and spleen at 1 hour after contusion in rats. With the extension of time after injury, the number of positive staining cells gradually increased and peaked at 12 hours The results of image analysis showed that the changes of IOD values of brain and heart, liver, lung, kidney and spleen were similar to those mentioned above, and the differences between the two groups were statistically significant There was a significant difference (P <0.05). Conclusion After brain contusion in rats, the expression of HSP70 in brain and vital organs and the same trend with time.