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目的研究1株临床分离产酸克雷伯菌中qnr基因新亚型的生物学特性及其多重耐药机制。方法克隆表达qnr新亚型基因,并用聚合酶链反应(PCR)检测β内酰胺酶基因和Ⅰ类整合酶基因的存在情况。结果qnr基因转化子对常见氟喹诺酮类抗菌药物的敏感性较受体菌下降3~25倍,但耐药水平低于临床分离菌株4~256倍。在产酸克雷伯菌中同时检出KLUC-1型超广谱β内酰胺酶(ESBLs)、TEM-1型和OXA-30型广谱β内酰胺酶基因。结论qnr基因可以轻度增加氟喹诺酮类药物的耐药性。携带qnr基因的临床菌株同时携带多种耐药基因。
Objective To study the biological characteristics of a novel subtype of qnr gene isolated from Klebsiella oxytoca and its multiple drug resistance mechanism. Methods The novel qnr gene was cloned and expressed, and the presence of β-lactamase gene and class Ⅰ integrase gene was detected by polymerase chain reaction (PCR). Results The sensitivity of qnr gene transformants to the common fluoroquinolone antibiotics was 3 ~ 25 times lower than that of the recipient bacteria, but the level of resistance was lower than that of the clinical isolates by 4 ~ 256 times. KLUC-1 ESBLs, TEM-1 and OXA-30-type broad-spectrum β-lactamase genes were also detected in Klebsiella oxytoca. Conclusion qnr gene can slightly increase the resistance of fluoroquinolones. Clinical strains carrying the qnr gene carry multiple resistance genes simultaneously.