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水稻籼粳亚种间杂种具有强大的生物学优势,但杂种F1普遍高度不育,难以直接利用.S5n基因可以克服由S5基因座位互作引起的杂种不育性.目前S5n基因已被成功克隆,该基因功能区存在大片段的缺失DNA,为快速并准确发掘携带有S5n的水稻新种质提供基础.本文将S5n基因序列与日本晴相对应序列进行比对,在S5n基因缺失DNA的两端设计引物,对已知携带有S5n和不携带有S5n基因(但携带有S5i或S5j)的水稻材料进行PCR扩增检测,建立S5n基因的功能性标记.利用该标记对我国水稻微核心种质进行检测,其中有10个品种的S5座位中存在DNA片段缺失,这些品种可能携带有S5n基因.这些品种包括毫补卡、小红谷、老造谷、三磅七十箩、木邦谷、魔王谷内杂、饭毫皮、飞蛾糯2、包协-7B和特青选恢等,其中除三磅七十箩和老造谷外,其余的8个品种均未见报道携带有S5n基因.对存在缺失DNA的10个品种的扩增片段进行测序,发现全部品种S5座位的缺失DNA片段都与02428和零轮(已知携带有S5n)的一致,说明这10个品种S5座位的基因也是没有功能的,证明确实存在S5n基因.
Intersubspecific hybrids between rice indica-japonica and subspecies have strong biological advantages, but F1 hybrids are generally highly infertile and difficult to be directly utilized.S5n gene can overcome the sterility caused by S5 locus interaction.S5n gene has been successfully cloned , There is a large fragment of the missing DNA in the functional region of the gene to provide a basis for rapid and accurate exploration of new rice germplasm carrying S5n.In this paper, we compared the S5n gene sequence with the corresponding sequence of Nipponbare, Primers were designed to detect the function of S5n gene by PCR amplification of rice material known to carry S5n and S5n gene (but carrying S5i or S5j) Of which there are 10 DNA fragments missing in the S5 locus of the 10 cultivars that may carry the S5n gene.These varieties include Millipore, Red Valley, Old Valley, three pound seventy, Wood State Valley, the devil Guzang miscellaneous, rice husks, moth waxy 2, Baosheng -7B and Teqing election restitution, in addition to three pounds seventy and old valley, the remaining eight species have not been reported to carry S5n gene. There are 10 varieties of DNA missing The results showed that all the missing DNA fragments of S5 locus were consistent with 02428 and zero round (known to carry S5n), indicating that the genes of S5 locus in these 10 cultivars were also non-functional, indicating that S5n gene.