In this research,a novel bird nest-like zinc oxide (BN-ZnO) nanostructures were prepared by a simple solvothermal method.A sensitive electrochemical glucose biosensor was for the first time developed based on the immobilization of glucose oxidase (GOx) on nanostructured BN-ZnO modified electrode.The BN-ZnO nanostructure and the resultant biosensor were characterized by scanning electron microscope,X-ray diffraction spectroscopy,Fourier transform infrared spectroscopy,and electrochemical impedance spectroscopy.BN-ZnO nanostructures have large specific surface area and can load large amounts of GOx molecules.Meanwhile,BN-ZnO provides an excellent microenvironment to retain the native bioactivity of enzymes and to promote direct electron transfer between GOx and electrode surface.The proposed biosensor shows a wide linear range of 0.005-1.6 mmol/L,high sensitivity of 15.6 mA L mo1-1 cm-2 with a low detection limit of 0.004 mmol/L.The resulting biosensor also shows excellent selectivity,acceptable stability and reproducibility,and can be successfully applied in the detection of glucose in human serum samples at-0.37 V.