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目的探讨儿童支气管哮喘(简称哮喘)发作期淋巴细胞免疫功能和白细胞介素-2(IL-2)、可溶性白介素2受体(sIL-2R)检测的临床意义。方法应用流式细胞术和酶联免疫吸附试验(ELISA)方法对25例支气管哮喘患儿和21例支气管哮喘缓解期患儿以及20例正常儿童外周血T细胞亚群及血清IL-2和sIL-2R水平进行测定。结果支气管哮喘患儿发作期血清sIL-2R水平明显高于缓解期和对照组,缓解期sIL-2R水平仍高于对照组。IL-2水平明显低于缓解期和对照组。外周血总CD3+、CD4+、CD4+/CD8+细胞比值均降低,CD8+细胞增高。结论动态监测IL-2和sIL-2R水平含量,观察T细胞亚群变化可以判断哮喘发作的程度、指导治疗和评估预后。
Objective To investigate the clinical significance of lymphocyte immune function, interleukin-2 (IL-2) and soluble interleukin-2 receptor (sIL-2R) detection in childhood bronchial asthma (asthma) attack. Methods T cell subsets, serum IL-2 and sIL in 25 children with bronchial asthma and 21 children with bronchial asthma with remission and 20 normal children were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA) -2R levels were measured. Results Serum levels of sIL-2R in children with bronchial asthma were significantly higher than those in remission and control groups, and sIL-2R levels in remission were still higher than those in control group. IL-2 levels were significantly lower than the remission and control group. Peripheral blood total CD3 +, CD4 +, CD4 + / CD8 + cells decreased, CD8 + cells increased. Conclusion The dynamic monitoring of IL-2 and sIL-2R levels, the observation of changes in T cell subsets can determine the extent of asthma attacks, and guide the treatment and assessment of prognosis.