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用大豆疫霉根腐病菌的游动孢子悬浮液处理抗感性不同的野生大豆下胚轴,接种后3～72 h,用透射电镜观察野生大豆与大豆疫霉菌的亲和性与非亲和性互作中细胞超微结构的变化。结果表明:接种后3～36 h,感病野生大豆随接种时间延长下胚轴细胞器结构破坏严重,而抗病野生大豆细胞结构基本完整;接种后48 h,抗病野生大豆的细胞壁物质累加,出现胞壁沉积物,而感病野生大豆细胞壁出现部分降解;接种后72 h,抗病野生大豆细胞质壁分离,但细胞器结构基本完整,感病野生大豆细胞器几乎无完整的结构。
Hypocotyls with different susceptibility to wild soybean were treated with zoospore suspension of Phytophthora sojae at 3 to 72 h after inoculation, and the compatibility and non-affinity of wild soybean to Phytophthora sojae were observed by transmission electron microscopy Changes in ultrastructure of cells during interaction. The results showed that the structure of hypocotyls was severely damaged in susceptible wild soybean with inoculation time 3 ~ 36 h after inoculation, while the cell structure of disease-resistant wild soybean was basically intact at 48 h after inoculation; the accumulation of cell wall substances in disease- While the cell wall of susceptible wild soybean showed partial degradation. At 72 h after inoculation, the cytoplasmic wall of disease-resistant wild soybean was separated, but the organelle structure was basically intact, and the susceptible wild soybean organelles had almost no intact structure.