论文部分内容阅读
目的探讨藤黄酸对人胃腺癌细胞株AGS的增殖和凋亡作用。方法用不同浓度藤黄酸处理AGS细胞后,MTT法测定AGS细胞的增殖活性,流式细胞术分析藤黄酸诱导AGS细胞凋亡,Western blot法检测B细胞淋巴瘤-白血病2(Bcl-2)和Bcl相关X蛋白(Bax)表达的变化。结果藤黄酸能剂量依赖性地抑制AGS细胞增殖、诱导AGS细胞凋亡、下调Bcl-2蛋白表达、上调Bax蛋白表达;24h半数抑制浓度(IC50)为0.9μmol/L。结论藤黄酸能明显抑制胃癌细胞株AGS的增殖,诱导AGS细胞凋亡。其促凋亡作用可能与Bcl-2、Bax的表达有关。
Objective To investigate the effects of gambogic acid on proliferation and apoptosis of human gastric adenocarcinoma cell line AGS. Methods After AGS cells were treated with different concentrations of gambogic acid, the proliferation of AGS cells was assayed by MTT assay. The apoptosis of AGS cells was analyzed by flow cytometry. The apoptosis of AGS cells was detected by Western blot. Bcl-2 ) And Bcl-related X protein (Bax) expression changes. Results Gambogic acid could inhibit AGS cell proliferation in a dose-dependent manner, induce apoptosis of AGS cells, down-regulate Bcl-2 protein expression and up-regulate the expression of Bax protein. The half inhibitory concentration (IC50) was 0.9μmol / L. Conclusion Gambogic acid can significantly inhibit the proliferation of gastric cancer cell line AGS and induce the apoptosis of AGS cells. Its role in promoting apoptosis may be related to the expression of Bcl-2 and Bax.