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目的探讨丁基苯酞对缺血性脑卒中大鼠工作记忆和皮质区Notch1表达的影响。方法 SD大鼠分为假手术组(对照组)、模型组、丁基苯酞高、低剂量组。参照改良的Pulsineli 4血管阻断建立大鼠缺血性脑卒中模型;HE染色观察各脑组织病理学形态的变化、免疫组化法检测Notch1的表达;八臂迷宫法和Morris水迷宫测试动物工作记忆情况。结果与对照组比较,脑缺血后皮质区神经元死亡数量、Notch1水平增加;八臂迷宫测试的动物工作记忆错误次数增多、水迷宫测试的穿台次数明显减少,差异有统计学意义(P<0.05)。与模型组比较,丁基苯酞组中大鼠脑缺血后海马区神经元死亡数量降低、Notch1水平增加;八臂迷宫测试的动物工作记忆错误次数减少、水迷宫测试的穿台次数明显增多,差异有统计学意义(P<0.05);上述变化在高剂量丁基苯酞组最为显著(P<0.05)。结论丁基苯酞可增加Notch1的表达、改善缺血性脑卒中大鼠工作记忆能力。
Objective To investigate the effect of butylphthalide on the expression of Notch1 in working memory and cortex in ischemic stroke rats. Methods SD rats were divided into sham operation group (control group), model group, butylphthalide high and low dose groups. The ischemic stroke model was established according to the improved Pulsineli 4 vascular occlusion. The pathological changes of brain tissue were observed by HE staining. The expression of Notch1 was detected by immunohistochemistry. The eight-arm maze and Morris water maze were used to test the animal work Memory situation. Results Compared with the control group, the number of neuronal deaths and the level of Notch1 in cortical neurons increased after cerebral ischemia; the number of working memory errors in the eight-arm maze test increased and the number of trauma in water maze test significantly decreased (P <0.05). Compared with the model group, the number of neuronal death in the hippocampus of butylphthalide group was decreased and the level of Notch1 increased. The number of erroneous working memory in the eight-arm maze test was decreased and the number of trauma test in water maze test was significantly increased , The difference was statistically significant (P <0.05); The above changes in the high-dose butylphthalide group was the most significant (P <0.05). Conclusion Butylphthalide can increase the expression of Notch1 and improve the working memory ability of ischemic stroke rats.